For years dermatophytes have posed serious problems in the area of both mycological diagnostics and therapy accurate and prompt identification of dermatophytes is necessary for clinical, epidemiological and preventive reasons it is not only the growing number of infections but also the species-related changes. Identification of common dermatophytes microscopic morphology of the micro and/or macroconidia is the most reliable identification character, but you need a good slide preparation and you may need to stimulate sporulation in some strains culture characteristics such as surface texture, topography and. The aim of the present study was to use two polymerase chain reaction (pcr) methods, with (gaca)4 and non‑transcribed spacer (nts) as primers, to identify and characterize dermatophyte isolates from dogs and cats to a species and strain level a total of 45 isolates from nine dermatophyte species were. Conclusions ap-pcr offers a convenient solution to the problems encountered in the recognition of morphological features of dermatophyte species in comparison with conventional morphological methods this approach represents technological progress in identification of dermatophytosis. Correlation of clinical manifestations of infection and observation of macro- and microscopic properties experienced technologists, morphological characteristics sterile mycelium (antifungal treatment) - dna sequence-based id (gold standard) expensive and time-consuming both restricted by slow growth of.
Full-text paper (pdf): isolation and identification of dermatophytes and other fungal agents from clinical specimens in erbil city. 1 monograph on dermatophytes a guide for isolation and identification of dermatophytes, diseases and treatment by mohamed refai, heidy abo el-yazid and mahmoud el-hariri department of microbiology, faculty of veterinary medicine cairo university 2013. This database has been built up to provide a tool for quick and accurate identification of dermatophytes using its sequences in its current version the database comprises over 200 sequences about 50% of the sequences belong to strains that are available from the cbs collection, while the remaining sequences have. Though resistance is uncommon (2), a proper identification of the infection agent is essential from an epidemiological point of view as well as for the choice of the antifungal treatment regimen and determining the source of infection (1) conventional identification of dermatophyte species relies mainly on the morphological.
Rapid detection of dermatophytes from skin and hair jaya garg, ragini tilak, atul gargemail author, pradyot prakash, anil kumar gulati and gopal nath bmc research notes20092:60 © garg et al licensee biomed central ltd 2009 received: 31 december 2008 accepted. Dermatophytes are associated with superficial infections in humans worldwide the aim of the present study was to determine the species distribution and susceptibility patterns of clinical dermatophytes samples received for routine mycological processing from 124 suspected cases attending a. The aim of this study was to evaluate matrix-assisted laser desorption ionization– time of flight mass spectrometry (maldi-tof ms) as a routine tool for identifying dermatophyte and neoscytalidium species, both of which cause dermatomycoses we first developed a spectral database of 12 different species of common and.
Rev 042913hh bacti-labtm skin culture systemstm - dermatophyte identification chart page 2 of 4 dermatophytes commonly seen in human and veterinary practice see list of terms above and refer to references (5-7) = of diagnostic importance microsporum canis microsporum gypseum microsporum nanum. Abstract the performance of a matrix-assisted laser desorption ionization– time of flight mass spectrometry (maldi-tof ms) workflow using an extensive reference database for dermatophyte identification was evaluated on 176 clinical strains using a direct-deposit procedure after 3 incubation days yielded 40% correct.
The conventional identification of dermatophytes requires a long turnaround time and highly skilled mycologists we have recently developed a standardized matrix-assisted laser desorption/ionization time-of-flight mass spectrometry ( maldi-tof ms) assay to routinely identify molds of potential clinical significance.
Identification of dermatophytes using the traditional method is sometimes problematic because of atypical microscopic or macroscopic morphology the aim of this study was to evaluate the feasibility of using sequencing of the ribosomal internal transcribed spacer (its)1 and its2 regions for identification of 17. Abstract: objective: phenotypic and genotypic identification of dermatophytes isolated from skin lesions of camels in this study 70 specimens of hair and skin scrapings were taken from camels with skin lesions from el – beheira governorate ( junbway / kom hamada / el – dalngat), el- giza governorate ( briqash ) and. Background dermatophyte identification is based on the detection of fungal elements by direct microscopy of clinical specimens combined with culture-based full identification phenotypic identification includes macromorphological, micromorphological, and physiological characteristics of the colonies in the last few years,. Abstract in this study we evaluated the suitability of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) for the identification of dermatophytes in diagnostic laboratories first, a spectral database was built with 108 reference strains belonging to 18 species of the anamorphic genera.